自然科学版 英文版
自然科学版 英文版
自然科学版 英文版

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中南大学学报(英文版)

Journal of Central South University

Vol. 14    No. 1    February 2007

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New amperometric glucose biosensor by entrapping glucose oxidase into chitosan/nanoporous ZrO2/multiwalled carbon nanotubes nanocomposite film
WEI Wan-zhi(魏万之)1, ZHAI Xiu-rong(翟秀荣)2, ZENG Jin-xiang(曾金祥)1, GAO Yan-ping(高艳萍)1, GONG Shu-guo(龚淑果)1

1. State key Laboratory of Chemo−Biosensing and Chemometrics, School of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, China;2. Department of Chemistry, Jining Teachers College, Jining 273155, China

Abstract:A new nanocomposite material for construction of glucose biosensor was prepared. The biosensor was formed by entrapping glucose oxidase(Gox) into chitosan/nanoporous ZrO2/multiwalled carbon nanotubes nanocomposite film. In this biosensing thin film, the multiwalled carbon nanotubes can effectively catalyze hydrogen peroxide and nanoporous ZrO2 can enhance the stability of the immobilized enzyme. The resulting biosensor provides a very effective matrix for the immobilization of glucose oxidase and exhibits a wide linear response range from 8 μmol/L to 3 mmol/L with a correlation coefficient of 0.994 for the detection of glucose. And the response time and detection limit of the biosensor are determined to be 6 s and 3.5 μmol/L, respectively. Another attractive characteristic is that the biosensor is inexpensive, stable and reliable.

 

Key words: biosensor; nanocomposite; glucose oxidase; nanoporous ZrO2; multiwalled carbon nanotubes; chitosan

中南大学学报(自然科学版)
  ISSN 1672-7207
CN 43-1426/N
ZDXZAC
中南大学学报(英文版)
  ISSN 2095-2899
CN 43-1516/TB
JCSTFT
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