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中南大学学报(自然科学版)

Journal of Central South University

第49卷    第10期    总第290期    2018年10月

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文章编号:1672-7207(2018)10-2636-07
微生物絮凝剂MBFA9对小球藻的沉降作用及其机理
张佳琪,曹琬,郑广泰,姜彬慧

(东北大学 资源与土木工程学院,辽宁 沈阳,110819)

摘 要: 以小球藻为研究对象,对絮凝菌类芽孢杆菌A9(Paebubacillus sp. A9)所产生的微生物絮凝剂MBFA9沉降水中微藻的性能进行研究。通过Zeta电位、结合键分析和扫描电镜表征探究MBFA9对小球藻的沉降机理。研究结果表明MBFA9对小球藻具有较好的沉降作用,其最佳沉降条件为:在小球藻对数生长期及平稳期添加Ca(OH)2调节pH至9.5,MBFA9添加量(体积分数)为0.2%(质量浓度为6 mg/L),在此最佳沉降条件下,沉降率为82.67%;随着pH升高,Zeta电位相应上升,在pH=9.5时达到最大;投加MBFA9后,分散状态的块状小球藻细胞迅速结合成网状结构,形成大的凝聚体而沉积,从而增大沉降速率。

 

关键字: 小球藻;pH;微生物絮凝剂;沉降作用

Harvesting of microbial flocculant MBFA9 on Chlorella vulgaris and its mechanism
ZHANG Jiaqi, CAO Wan, ZHENG Guangtai, JIANG Binhui

School of Resources and Civil Engineering, Northeastern University, Shenyang 110819, China

Abstract:The characteristic and mechanism of harvesting Chlorella vulgaris by microbial flocculant MBFA9 which was produced by flocculation bacteria (Paebubacillus sp. A9) were studied. The sedimentation mechanism of Chlorella vulgaris by MBFA9 was investigated by Zeta potential, binding bond analysis and scanning electron microscopy. The results show that the sedimentation rate is 82.67% under the optimum settling conditions, i.e. adjusting pH=9.5 by adding Ca(OH)2 during exponential growth period and stationary period,and the additive volume fraction of MBFA9 is 0.1%. With the increase of pH, the Zeta potential increases accordingly and reaches the maximum when pH=9.5. After adding MBFA9, the dispersing state of Chlorella vulgaris cells combine rapidly into a network structure to form large agglomerates and deposite, leading to the increase of the sedimentation rate.

 

Key words: Chlorella vulgaris; pH; microbial flocculant; harvesting

中南大学学报(自然科学版)
  ISSN 1672-7207
CN 43-1426/N
ZDXZAC
中南大学学报(英文版)
  ISSN 2095-2899
CN 43-1516/TB
JCSTFT
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